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Why does hydrogen peroxide plasma sterilization fail in low temperature?
2022-05-21

Hydrogen peroxide low temperature plasma sterilizer is suitable for sterilization of medical instruments and articles that are not heat-resistant and moisture-resistant, such as various lenses used in operating rooms, slender catheters for clinical use, and slender inner tubes with slender lumen structures. speculum etc. It has the advantages of strong penetration, low temperature resistance, short sterilization time, safety and reliability.

1. Working principle
Under the condition of low temperature and vacuum, plasma is generated by the action of high-frequency electric field in the sterilization chamber, in which the active substance chemically reacts with the protein and nucleic acid in the microorganism, destroying the microorganism and disturbing the survival function of the microorganism, and then using H²O² as a medium to act In microbial cells, the microorganisms are further killed.
There are many factors affecting the sterilization in the low temperature plasma sterilization process. If it is not handled properly, it will lead to sterilization failure, which not only increases the workload but also wastes time.
2. Reasons for failure and countermeasures
Incomplete cleaning of instruments: blood and other impurities remain on the instruments, which will affect the sterilization of the low-temperature plasma sterilizer.
Before sterilization, the instruments should be thoroughly cleaned in accordance with the relevant provisions of the "Hospital Disinfection Supply Center Cleaning and Disinfection Technical Operation Specifications".
Insufficient drying of instruments: Hydrogen peroxide is soluble in water, and if the instrument is wet, it will consume too much hydrogen peroxide in the sterilization chamber, reduce the concentration, and cause sterilization failure.
Check the cleanliness and dryness of the items to be sterilized, and pack them individually in sterile packaging bags, or put them in a sterilized instrument box and wrap them with double-layer non-woven fabrics.
Improper loading of instruments during sterilization: stacking of instruments, excessive length of instruments, or contact with bulkhead doors can all affect.
The sterile packaging bags filled with instruments should be placed one by one in a single layer, and should not be overlapped. Metal instruments should not be in contact with the side wall of the sterilization chamber, and the distance from the wall of the sterilization chamber should be more than 5cm.
The own factors of the sterilization system: sterilization failure due to the failure of the equipment's own system.
Before sterilization, check whether the equipment is in good condition and set the running program. During the sterilization process, carefully observe the temperature displayed on the monitoring screen and the operation status of the machine. If there is a fault, stop the sterilization immediately.
Unqualified or expired: should be stored at room temperature in a cool and dark place.
The staff should replace the hydrogen peroxide liquid in time, and mark the replacement date.
The indicator card does not change color: if the indicator card is placed close to the plastic surface of the packaging bag, and the hydrogen peroxide cannot touch the indicator card discoloration strip during the sterilization process, the indicator card will not change color, or the indicator card will be placed in the air for time. If it is too long, it has been oxidized or become damp even within the validity period, and it will not change color after sterilization.
The indicator card should be stored in the dark to prevent oxidation. When using it, use tweezers to gently pick it up and put it in the packaging bag to prevent pollution.
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